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biotinylated goat anti rat antiserum  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti rat antiserum
    Biotinylated Goat Anti Rat Antiserum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+rat+antiserum/Biotinylated+Goat+Anti-Rat+IgG+Antibody/pmc02775944-56-4-9
    Average 96 stars, based on 2112 article reviews
    biotinylated goat anti rat antiserum - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Alzheimer’s-Type Amyloidosis in Transgenic Mice Impairs Survival of Newborn Neurons Derived from Adult Hippocampal Neurogenesis
    Article Snippet: Sections were then incubated overnight at room temperature in a mixture of anti-BrdU antibody (1:800), monoclonal mouse anti-NeuN (1: 5000, MAB377; Chemicon, Temecula, CA), and rabbit anti-S100 β (1: 5000, 37a; Swant, Bellinzona, Switzerland) in PBST-Az containing 5% NGS. .. The next day, sections were rinsed several times in PBST before being incubated for 90 min at room temperature in a mixture of secondary reagents: biotinylated goat anti-rat antiserum (1:400; Vector Laboratories), Alexa 488-conjugated highly cross-adsorbed goat anti-mouse IgG (1:250; Invitrogen, Carlsbad, CA), and Alexa 647-conjugated goat anti-rabbit IgG (1:250; Invitrogen) in PBST. .. Sections were rinsed again and finally incubated in streptavidin–tetramethylrhodamine isothiocyanate (TRITC; 1:1000 in PBST; Beckman Coulter, Fullerton, CA).

    Article Title: Alzheimer’s-Type Amyloidosis in Transgenic Mice Impairs Survival of Newborn Neurons Derived from Adult Hippocampal Neurogenesis
    Article Snippet: Sections were blocked in PBST with 5% NGS for 30 min, followed by overnight incubation at room temperature in monoclonal rat anti-BrdU (OBT-0030; Harlan Seralab, Loughborough, UK) diluted 1:400 in PBST-Az and 5% NGS. .. The next day, sections were rinsed several times in PBST and incubated for 90 min at room temperature in biotinylated goat anti-rat antiserum (Vector Laboratories) diluted 1:400 in PBST. .. Sections were again rinsed with PBST before being incubated for 90 min at room temperature in avidin–biotin–peroxidase complex (Elite kit; Vector Laboratories) diluted 1:400 in PBST.

    Article Title: Alzheimer's-Type Amyloidosis in Transgenic Mice Impairs Survival of Newborn Neurons Derived from Adult Hippocampal Neurogenesis
    Article Snippet: Sections were blocked in PBST with 5% NGS for 30 min, followed by overnight incubation at room temperature in monoclonal rat anti-BrdU (OBT-0030; Harlan Seralab, Loughborough, UK) diluted 1:400 in PBST-Az and 5% NGS. .. The next day, sections were rinsed several times in PBST and incubated for 90 min at room temperature in biotinylated goat anti-rat antiserum (Vector Laboratories) diluted 1:400 in PBST. .. Sections were again rinsed with PBST before being incubated for 90 min at room temperature in avidin– biotin–peroxidase complex (Elite kit; Vector Laboratories) diluted 1:400 in PBST.

    Article Title: Inhibition of PI3K-Akt Signaling Blocks Exercise-Mediated Enhancement of Adult Neurogenesis and Synaptic Plasticity in the Dentate Gyrus
    Article Snippet: For immunohistofluorescence, sections were hydrolyzed as described above, followed by overnight incubation in monoclonal rat anti-BrdU (1∶400, OBT0030; AbCys). .. Sections were incubated in biotinylated goat anti-rat antiserum (1∶400; Vector Laboratories), then in streptavidin Alexa-568 (1∶800; Invitrogen, Eugene, OR). .. Sections were then incubated overnight in mouse monoclonal anti-NeuN (1∶500; Chemicon), in Alexa-488 goat anti-mouse highly cross-adsorbed antiserum (1∶200; Molecular Probes) and counterstained with DNA dye bisbenzimid (Hoechst 33342, Sigma; 1 ug/mL).



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